Title :
Multi-stage, solvent-controlled routing for automated on-disc extraction of total RNA from breast cancer cell line homogenate
Author :
Gaughran, J. ; Dimov, N. ; Clancy, E. ; Barry, T. ; Smith, T.J. ; Ducree, J.
Author_Institution :
Biomed. Diagnostics Inst., Dublin City Univ., Dublin, Ireland
Abstract :
We report for the first time rotationally controlled extraction of total RNA from cell homogenate through novel, solvent-dependent routing on a centrifugal micro-fluidic platform. This system allows for sample preparation and integrated analysis to be incorporated on a single microfluidic device. Also reported here for the first time is a solvent-triggered valving mechanism and the performance of on-disc extraction of purified total RNA.
Keywords :
RNA; biomedical engineering; cancer; cellular biophysics; microfluidics; automated on-disc extraction; breast cancer cell line homogenate; centrifugal microfluidic platform; multistage routing; single microfluidic device; solvent-controlled routing; solvent-triggered valving mechanism; total RNA; Educational institutions; Liquids; Microfluidics; Protocols; RNA; Routing; Valves; Total RNA extraction; centrifugal microfluidics; solvent dependent routing; solvent triggered valving;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on
Conference_Location :
Barcelona
DOI :
10.1109/Transducers.2013.6626763
