Frequency dependence of tissue attenuation measured by acoustic microscopy

The authors have developed a method of acoustic time-domain reflectometry that allows the elastic properties of a histological section to be measured with a resolution of around 8 μm. Experimental data obtained using this technique have been processed so that the frequency dependence of the tissue attenuation is revealed. Comparisons are also made between scanning acoustic microscope (SAM) and SLAM data for similar tissue samples. Observations are made on the lower leg muscles of an adult ICR mouse. An analysis is made of a toneburst SAM image obtained with the lens focused on the surface of the microscope slide, at a frequency of 425 MHz and a field of view of 425 μm