Title :
2D cantilever array with spherical tips for parallel force spectroscopy on cancerous cells
Author :
Loizeau, F. ; Favre, M. ; Akiyama, Toyokazu ; Gautsch, S. ; Meister, A. ; Smajda, R. ; Vettiger, P. ; Weder, G. ; de Rooij, Nico F.
Author_Institution :
Ecole Polytech. Fed. de Lausanne (EPFL), Lausanne, Switzerland
Abstract :
The nanomechanical properties of cancerous cells are of great interest to understand how a localized tumor becomes metastatic. We present a 2D cantilever array to investigate the stiffness and adhesion characteristics of multiple cells at a high throughput. A new molding process based on thermal oxidation of silicon allows us to create at a wafer scale spherical tips with a radius of curvature of 3.8 μm. Simulations show that radii ranging from 2.5 μm to 6.5 μm can be produced. The customized cantilever array system was used to simultaneously probe 13 melanoma living cells (WM239). The Young´s moduli of the cells were successfully obtained with the Hertz model.
Keywords :
Young´s modulus; adhesion; bioMEMS; biomechanics; biomedical optical imaging; cancer; cantilevers; cell motility; elastic constants; microfabrication; silicon; tumours; 2D cantilever array; Hertz model; Si; WM239; Young´s moduli; cancerous cells; curvature radius; customized cantilever array system; localized tumor; melanoma living cell; metastatic tumor; molding process; multiple cell adhesion characteristics; multiple cell stiffness; nanomechanical properties; parallel force spectroscopy; radius 2.5 mum to 6.5 mum; radius 3.8 mum; thermal oxidation; wafer scale spherical tips; Arrays; Cancer; Etching; Oxidation; Probes; Shape; Silicon; AFM; cantilever array; melanoma cells; microfabrication; parallel force spectroscopy; spherical tips;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on
Conference_Location :
Barcelona
DOI :
10.1109/Transducers.2013.6626985
