On-demand electrophoretic separation of DNA in written gel lines on planar substrates

Author

Tanguy, Laurent ; Gutzweiler, Ludwig ; Koltay, Peter ; Zengerle, Roland ; Riegger, Lutz

Author_Institution

HSG-IMIT, Freiburg, Germany

fYear

2013

fDate

16-20 June 2013

Firstpage

1223

Lastpage

1226

Abstract

We report a new approach to perform on-demand electrophoretic separation of DNA. In contrast to standard chip-based capillary electrophoresis in micromachined glass chips, we apply a planar polyimide substrate, write 200 μm wide gel lines bridging two Pt-electrodes and inject 500 pl sample volumes in non-contact manner. The gel is covered with mineral oil to inhibit evaporation. Subsequently, an electrical field is applied for 80 s and the separation of the DNA molecules (56 bp-Cy5 and 112 bp-Cy5, 10 μM) is successfully demonstrated.

Keywords

DNA; biochemistry; bioelectric phenomena; biological techniques; electrochemical electrodes; electrophoresis; evaporation; gels; molecular biophysics; oils; platinum; polymers; separation; DNA molecule electrophoretic separation; Pt; chip-based capillary electrophoresis; electrical field; evaporation; gel line; micromachined glass chip; mineral oil; planar polyimide substrate; platinum electrode; size 200 mum; time 80 s; DNA; Electrodes; Liquids; Microfluidics; Printing; Substrates; Writing; DNA separation; Electrophoresis; light induced fluorescence detection; open microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on

Conference_Location

Barcelona

Type

conf

DOI

10.1109/Transducers.2013.6626995

Filename

6626995