Title :
Determination of glucose and albumin in urine using Mid-Infrared Attenuated Total Reflection System
Author :
Liu, Weiling ; Liu, Libing ; Xu, Kexin ; Yu, Qilian
Author_Institution :
Hebei Univ. of Technol., Tianjin
Abstract :
A reagent-free method applied for component analysis of glucose and albumin in urine which using ATR spectroscopy was studied. Concentration of glucose and albumin have significant mid-infrared absorption bands. They are related to the molecule vibration of CH, NH, CO or CC. The calibration model of glucose and albumin in urine have been established using attenuated total reflectance (ATR) Mid-infrared spectroscopy combined with partial least squares (PLS) regression. Compared with biochemical method, this method has provided with advantages such as high- speed, simplicity and good repeatability. RMSEP (Root Mean Square Error of Prediction) for glucose and albumin is 20.5 mg/dL and 17.8 mg/dL respectively, and the correlation is 0.999 and 0.998. The result method have the potential to serve as an effective addition to the clinical chemistry laboratory
Keywords :
biochemistry; biomedical measurement; chemical variables measurement; infrared spectroscopy; mean square error methods; spectrochemical analysis; albumin determination; biochemical method; calibration model; component analysis; glucose concentration determination; mid-infrared attenuated total reflection spectroscopy system; molecule vibration; partial least squares regression; reagent-free method; root mean square error for prediction; urine; Absorption; Calibration; Chemistry; Laboratories; Least squares methods; Reflection; Reflectivity; Root mean square; Spectroscopy; Sugar; ATR; Mid-Infrared spectroscopy; Partial Least Squares (PLS); components in urine;
Conference_Titel :
Information Acquisition, 2006 IEEE International Conference on
Conference_Location :
Weihai
Print_ISBN :
1-4244-0528-9
Electronic_ISBN :
1-4244-0529-7
DOI :
10.1109/ICIA.2006.305928
