Determination of glucose and albumin in urine using Mid-Infrared Attenuated Total Reflection System

A reagent-free method applied for component analysis of glucose and albumin in urine which using ATR spectroscopy was studied. Concentration of glucose and albumin have significant mid-infrared absorption bands. They are related to the molecule vibration of CH, NH, CO or CC. The calibration model of glucose and albumin in urine have been established using attenuated total reflectance (ATR) Mid-infrared spectroscopy combined with partial least squares (PLS) regression. Compared with biochemical method, this method has provided with advantages such as high- speed, simplicity and good repeatability. RMSEP (Root Mean Square Error of Prediction) for glucose and albumin is 20.5 mg/dL and 17.8 mg/dL respectively, and the correlation is 0.999 and 0.998. The result method have the potential to serve as an effective addition to the clinical chemistry laboratory