Title :
Precise control of DNA orientation for improved functionlity in protein binding microarrays
Author :
Spuhler, P. ; Xirui Zhang ; Monroe, Megan ; Greenspun, J. ; Unlu, M.S. ; Sola, L. ; Chiari, M.
Author_Institution :
Biomed. Eng., Boston Univ., Boston, MA, USA
Abstract :
We demonstrate controlled orientation of surface anchored DNA and utilize spectral self-interference fluorescent microscopy (SSFM) to measure fluorophore height with sub-nm precision to precisely quantify DNA orientation and conformation.
Keywords :
DNA; fluorescence; molecular biophysics; molecular configurations; proteins; DNA orientation; fluorophore height; protein binding microarrays; spectral self-interference fluorescent microscopy; surface anchored DNA; DNA; Fluorescence; Polymers; Probes; Protein engineering; Proteins; DNA biosensor; bioNEMS; protein binding microarrays;
Conference_Titel :
Optical MEMS and Nanophotonics (OMN), 2011 International Conference on
Conference_Location :
Istanbul
Print_ISBN :
978-1-4577-0334-8
DOI :
10.1109/OMEMS.2011.6031084
