Plate reader compatible membrane-integrated microfluidic platform for high-throughput cellular assays

Author

Vereshchagina, E. ; McGlade, D. ; Glynn, M. ; Ducree, J.

Author_Institution

Nat. Centre for Sensor Res., Dublin City Univ., Dublin, Ireland

fYear

2013

fDate

16-20 June 2013

Firstpage

2177

Lastpage

2180

Abstract

This work reports a novel, versatile, and membrane-integrated 3-dimensional microfluidic platform (MP) for high-throughput biodiagnostics, with an aim towards applications in clinical cancer studies. The hybrid MP is designed to be compatible with standard equipment used in high throughput screening, featuring membrane supported micro-wells and gradient generating microstructures to enable exposure of cell samples to various concentrations of reagents of interest, suitable to run both fluorometric and colorimetric assays. This paper discusses design and fabrication of the MP, and also describes preliminary testing of colorimetric and fluorometric assays carried out on the device.

Keywords

cellular biophysics; colorimetry; membranes; microfluidics; radiography; 3-dimensional microfluidic platform; clinical cancer studies; colorimetric assays; fluorometric assays; gradient generating microstructures; high-throughput biodiagnostics; high-throughput cellular assays; membrane supported microwells; plate reader compatible membrane-integrated microfluidic platform; Biomembranes; Drugs; Microfluidics; Prototypes; Standards; Throughput; Microfluidic platform; cell; high-throughput assay; polycarbonate membrane; polymer rapid prototyping;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on

Conference_Location

Barcelona

Type

conf

DOI

10.1109/Transducers.2013.6627234

Filename

6627234