Title :
Lung cancer model on chip for drug testing
Author :
Kuo-Wei Chang ; Punde, Tushar Harishchandra ; Pendharkar, Gaurav Prashant ; Po-Chen Shih ; Yao-Fei Chan ; Kang-Yun Lee ; Ming-Yan Chen ; Cheng-Hsien Liu
Author_Institution :
Dept. of Power Mech. Eng., Nat. Tsing Hua Univ., Hsinchu, Taiwan
Abstract :
In this paper we have simulated an in vitro lung cancer microenvironment by dielectrophoresis (DEP) patterning. Heterogeneous cells were patterned on the glass substrate, above which a microfluidic gradient is integrated to generate different concentration of target therapy drug (Iressa). Taking advantage of DEP pattern and microfluidic gradient, we designed a biochip to mimic the in vivo conditions. The influence of drug treatment for cell patterning is better than the non-patterned case as visualized in cell viability rate by MTT assay.
Keywords :
cancer; cellular biophysics; electrophoresis; lab-on-a-chip; lung; microfluidics; biochip; cell patterning; dielectrophoresis patterning; drug testing; drug treatment; glass substrate; heterogeneous cells; in vitro lung cancer microenvironment; lung cancer model; microfluidic gradient; target therapy drug; Cancer; Drugs; Electrodes; Fibroblasts; Glass; Lungs; Substrates; Dielectrophoresis; Gradient Microchannel; Lung Cancer Patterned; Target Therapy Drug;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on
Conference_Location :
Anchorage, AK
DOI :
10.1109/TRANSDUCERS.2015.7181006