Title :
Label-free and ultrasensitive detection of microrna biomarkers in lung cancer cells based on silicon nanowire FET biosensors
Author :
Gao, Amy ; Lu, Ning ; Dai, P. ; Li, Tong ; Wang, Yannan
Author_Institution :
State Key Labs. of Transducer Technol., Shanghai Inst. of Microsyst. & Inf. Technol., Shanghai, China
Abstract :
MicroRNAs (miRNAs) have been regarded as biomarkers for early-stage diagnosis of cancer. Due to their short length, low abundance, and very similar sequences, an attractive approach that is ultrasensitive and label-free is extremely demanded. Here we report the design of a novel semiconducting silicon nanowire field-effect transistor (SiNW-FET) biosensor array for ultrasensitive label-free and real-time detection of miR-21. Optical lithography and anisotropic self-stop etching were employed to guarantee low cost batch production for SiNWs. The working conditions of the nanosensor were firstly optimized through pH sensing experiment. This SiNW-FET nanosensor under optimal conditions revealed ultrahigh sensitivity for repaid and reliable detection of 1 fM miR-21 and high specificity for single-nucleotide polymorphism discrimination.
Keywords :
RNA; biomedical materials; biosensors; cancer; cellular biophysics; etching; field effect transistors; lung; molecular biophysics; nanomedicine; nanosensors; nanowires; pH; photolithography; semiconductor devices; silicon; 1 fM miR-21 detection; SiNW-FET biosensor array design; SiNW-FET nanosensor; anisotropic self-stop etching; early-stage cancer diagnosis; high sensor specificity; label-free detection; low cost SiNW batch production; low miRNA abundance; lung cancer cell; miR-21 real-time detection; miRNA sequence; microRNA biomarker; nanosensor working condition; optical lithography; pH sensing experiment; semiconducting silicon nanowire field-effect transistor; short miRNA length; silicon nanowire FET biosensor; single-nucleotide polymorphism; ultrasensitive detection; Cancer; DNA; Logic gates; Nanobioscience; Sensors; Silicon; Surface treatment; SiNWs-FET; label-free; miRNA; ultrasensitive;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on
Conference_Location :
Barcelona
DOI :
10.1109/Transducers.2013.6627299
