Title :
The cell detection using EIS sensor and phage display
Author :
Yunfang, Jia ; Wencheng, Niu ; Xiao, Li ; Guohua, Liu ; Hongkai, Zhang ; Youjia, Cao ; Xizeng, Fen ; Ming, Qin
Author_Institution :
Dept. of Microelectron., Nankai Univ., Tianjin
Abstract :
A new method for cell recognition by using electrolyte-insulator-semiconductor (EIS) structure sensor, the light-addressable potentiometric sensor (LAPS), integrated with a bio-sensitive membrane is elaborated. The structure of LAPS is Si3 N4/SiO2/Si. And the phage probe specific to the transferring breast cancer cell, MDAMB231, which is prepared by phage display is used as the bio-sensitive membrane. This membrane is immobilized on the surface of Si3N4 by the covalent binding. Then this phage-LAPS is used to detect the MDAMB231 in the solution, the concentrations of which are 5 times 103/mL, 5 times 10/mL, 5 times 105/mL
Keywords :
biosensors; cancer; silicon compounds; EIS sensor; Si3N4-SiO2-Si; bio-sensitive membrane; cell detection; covalent binding; electrolyte-insulator-semiconductor structure sensor; light-addressable potentiometric sensor; phage display; Biomembranes; Biosensors; Circuits; Displays; Electrodes; Peptides; Probes; Semiconductor lasers; Sensor phenomena and characterization; Sensor systems;
Conference_Titel :
Solid-State and Integrated Circuit Technology, 2006. ICSICT '06. 8th International Conference on
Conference_Location :
Shanghai
Print_ISBN :
1-4244-0160-7
Electronic_ISBN :
1-4244-0161-5
DOI :
10.1109/ICSICT.2006.306350
