The cell detection using EIS sensor and phage display

Author

Yunfang, Jia ; Wencheng, Niu ; Xiao, Li ; Guohua, Liu ; Hongkai, Zhang ; Youjia, Cao ; Xizeng, Fen ; Ming, Qin

Author_Institution

Dept. of Microelectron., Nankai Univ., Tianjin

fYear

2006

fDate

23-26 Oct. 2006

Firstpage

551

Lastpage

553

Abstract

A new method for cell recognition by using electrolyte-insulator-semiconductor (EIS) structure sensor, the light-addressable potentiometric sensor (LAPS), integrated with a bio-sensitive membrane is elaborated. The structure of LAPS is Si₃N₄/SiO₂/Si. And the phage probe specific to the transferring breast cancer cell, MDAMB231, which is prepared by phage display is used as the bio-sensitive membrane. This membrane is immobilized on the surface of Si₃N₄ by the covalent binding. Then this phage-LAPS is used to detect the MDAMB231 in the solution, the concentrations of which are 5 times 10³/mL, 5 times 10/mL, 5 times 10⁵/mL

Keywords

biosensors; cancer; silicon compounds; EIS sensor; Si₃N₄-SiO₂-Si; bio-sensitive membrane; cell detection; covalent binding; electrolyte-insulator-semiconductor structure sensor; light-addressable potentiometric sensor; phage display; Biomembranes; Biosensors; Circuits; Displays; Electrodes; Peptides; Probes; Semiconductor lasers; Sensor phenomena and characterization; Sensor systems;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State and Integrated Circuit Technology, 2006. ICSICT '06. 8th International Conference on

Conference_Location

Shanghai

Print_ISBN

1-4244-0160-7

Electronic_ISBN

1-4244-0161-5

Type

conf

DOI

10.1109/ICSICT.2006.306350

Filename

4098163