Isothermal real-time polymerase chain reaction detection of Herpes Simplex Virus Type 1 on a light-actuated digital microfludics platform

Author

ShaoNing Pei ; Yi-Lun Wang ; Chih-Ting Lin ; Wu, Ming C.

Author_Institution

Berkeley Sensor & Actuator Center & Electr. Eng. & Comput. Sci., Univ. of California, Berkeley, Berkeley, CA, USA

fYear

2013

fDate

16-20 June 2013

Firstpage

2791

Lastpage

2794

Abstract

We report on a light-actuated digital microfluidics platform for real-time, isothermal helicase-dependent polymerase chain reaction (PCR). This platform is used to detect Herpes Simplex Virus Type 1 (HSV-1). Reagents in droplets are individually addressed, mixed, transported and arrayed on-chip before the PCR process. 16 droplets were formed from mixing 400 nl PCR master mix droplets with 35 nl HSV-1 viral lysate droplets, arrayed into a 4×4 matrix, and amplified simultaneously, all on chip. Amplification and detection of HSV-1 can be achieved within 45 minutes. No cross contamination was observed.

Keywords

bioMEMS; biochemistry; diseases; drops; lab-on-a-chip; microactuators; microfluidics; microorganisms; patient diagnosis; real-time systems; HSV-1 amplification; HSV-1 detection; HSV-1 viral lysate droplet; Herpes Simplex Virus Type 1; PCR master mix droplet; arrayed on-chip; isothermal real-time polymerase chain reaction detection; light-actuated digital microfludics platform; mixing; time 45 min; Arrays; Fluorescence; Isothermal processes; Microfluidics; Polymers; Real-time systems; System-on-chip; Digital Microfluidics; EWOD; Electrowetting-on-Dielectric; Herpes Simplex Virus; Isothermal Polymerase Chain Reaction; Light-Actuated Digital Microfluidics; Polymerase Chain Reaction;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on

Conference_Location

Barcelona

Type

conf

DOI

10.1109/Transducers.2013.6627385

Filename

6627385