DocumentCode :
346709
Title :
Inhibition of intestinal proteases decreases cellular activation in SAO shock
Author :
Mitsuoka, H. ; Kistler, E. ; Schmid-Schönbein, G.W.
Author_Institution :
Dept. of Bioeng., California Univ., San Diego, La Jolla, CA, USA
Volume :
1
fYear :
1999
fDate :
1999
Abstract :
Splanchnic arterial occlusion (SAG) shock is accompanied by upregulated levels of cellular activation. Pancreatic proteases as well as proteolytically derived substances may constitute primary activators, turning the small intestine into a production site for activators. The pathophysiological role of intestinal proteases in rat´s SAO shock was investigated. Duodenum and terminal ileum were cannulated with polyethylene tubings, and the intestine was flushed with saline. Using a peristaltic pump, the intestine was continuously circulated by 50 ml of saline with/without 10 mg of protease inhibitor, Nafamostat mesilate (FUT-175). SAO shock was induced by clamping the superior mesenteric and celiac arteries for 100 min, followed by reperfusion. Rats in FUT-175 perfused group had significantly lower production of activator, measured by pseudopod formation on naive donor leukocytes exposed to portal venous plasma film shocked animals (0.01). Inhibition of intestinal proteases could keep the mean arterial pressure 89% of baseline in average. These results indicate intestinal proteases play an important role in the production of the primary activators for circulating leukocytes and endothelial cells in SAO shock
Keywords :
biological organs; blood vessels; cellular transport; diseases; molecular biophysics; proteins; Nafamostat mesilate perfused group; cellular activation; circulating leukocytes; duodenum; endothelial cells; intestinal proteases; mean arterial pressure; naive donor leukocytes; pancreatic proteases; pathophysiological role; peristaltic pump; polyethylene tubings; portal venous plasma film shocked animals; primary activators; production site; protease inhibitor; proteolytically derived substances; pseudopod formation; rat splanchnic arterial occlusion shock; reperfusion; saline; small intestine; superior celiac arteries; superior mesenteric arteries; terminal ileum; upregulated levels; Arterial occlusion; Electric shock; Inhibitors; Intestines; Pancreas; Plasma measurements; Polyethylene; Production; Turning; White blood cells;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
ISSN :
1094-687X
Print_ISBN :
0-7803-5674-8
Type :
conf
DOI :
10.1109/IEMBS.1999.802045
Filename :
802045
Link To Document :
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