Title :
Effects of shear stress on tissue factor gene expression in endothelial cells
Author :
Lin, Kurt ; Shyy, John Y -J ; Chien, Shu
Author_Institution :
Dept. of Bioeng. & Inst. of Biomed. Eng., California Univ., San Diego, La Jolla, CA, USA
Abstract :
Studying the effects of shear stress on the expression of tissue factor (TF) gene in cultured endothelial cells (ECs), the authors found that a GC-rich region of the promoter that contains EGR-1 and Sp1 sites is responsible for the shear stress induction of TF gene. The increased transcriptional activity is mediated through a novel mechanism of Sp1 phosphorylation, rather than DNA binding
Keywords :
biological tissues; biomechanics; cellular biophysics; genetics; DNA binding; EGR-1 sites; Sp1 phosphorylation; Sp1 sites; endothelial cells; increased transcriptional activity; promoter GC-rich region; shear stress effects; tissue factor gene expression; Biomedical engineering; Blood; Cells (biology); Coagulation; DNA; Gene expression; Genetic mutations; In vivo; Region 1; Stress;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.802067
