Label-free detection of leukemia cells with a lab-on-a-chip system integrating dielectrophoresis and cmos imaging

Author

Demircan, Y. ; Orguc, S. ; Musayev, J. ; Ozgur, E. ; Erdem, M. ; Gunduz, U. ; Eminoglu, S. ; Kulah, H. ; Akin, T.

Author_Institution

Dept. of Electr. & Electron. Eng., METU, Ankara, Turkey

fYear

2015

fDate

21-25 June 2015

Firstpage

1589

Lastpage

1592

Abstract

This paper presents a fully-integrated lab-on-a-chip (LOC) system for label-free detection and real-time counting of dielectrophoretically trapped multidrug resistant (MDR) K562 cells. The system integrates a parylene-based microfluidic DEP channel on top of a CMOS image sensor for the first time in the literature. The DEP channel can trap MDR K562 cells with 9 Vpp and 10 μl/min flow rate, and the CMOS image sensor can detect the trapped cells as small as 3 μm in diameter with a noise level of 28.3 e-rms.

Keywords

CMOS image sensors; bioMEMS; bioelectric phenomena; biomedical equipment; biomedical optical imaging; blood; cancer; cellular biophysics; drugs; electrophoresis; lab-on-a-chip; microchannel flow; polymers; real-time systems; CMOS image sensor; CMOS imaging; MDR K562 cell trapping; dielectrophoresis; dielectrophoretically trapped K562 cell diameter; fully-integrated LOC system; lab-on-a-chip system; label-free leukemia cell detection; multidrug resistant K562 cell; noise level; parylene-based microfluidic DEP channel; real-time K562 cell counting; CMOS image sensors; CMOS integrated circuits; Cancer; Dielectrophoresis; Electrodes; Microfluidics; CMOS; Lab-on-a-chip (LOC); dielectrophoresis (DEP); label-free; multidrug resistance (MDR);

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181243

Filename

7181243