Title :
Culture of osteoblastic cells in porous scaffolds with convection
Author :
Goldstein, Aaron S. ; Mikos, A.G.
Author_Institution :
Dept. of Bioeng., Rice Univ., Houston, TX, USA
Abstract :
One approach for the repair of bone defects is the use of porous biodegradable scaffolds seeded with osteoblastic cells. Proliferation of these cells may be limited by diffusion rates of nutrients. Therefore, culture conditions that convect media may enhance proliferation. In addition, shear stresses associated with convection may enhance osteoblastic function of the cells. In this study four culture systems are examined: static culture, rotary vessel, spinner flask, and perfusion system. Cell density, cell distribution within the foam and osteoblastic function of seeded cells are characterized to ascertain the effect of culture systems on osteoblastic cell growth in 75:25 poly(DL-lactic-co-glycolic acid) (PLGA) foams
Keywords :
biodiffusion; biological techniques; bone; cellular biophysics; convection; foams; bone defects repair; cell density; cell distribution within foam; cell growth; chemotaxis; convection; culture conditions; diffusion rates of nutrients; osteoblastic cells; osteoblastic function; perfusion system; poly(DL-lactic-co-glycolic acid) foams; porous biodegradable scaffolds; rotary vessel; seeded cells; shear stresses; spinner flask; static culture; Adhesives; Biodegradable materials; Biological materials; Bone tissue; Cells (biology); Chemical engineering; Engine cylinders; Mineralization; Shearing; Stress;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.802148
