Title :
Modulation of osteoblastic phenotype by substrate-dependent changes in fibronectin conformation
Author :
Keselowsky, B.G. ; Byers, B.A. ; García, A.J.
Author_Institution :
Dept. of Biomed. Eng., Georgia Inst. of Technol., Atlanta, GA, USA
Abstract :
Adsorption of fibronectin onto untreated and tissue culture grade polystyrenes and collagen type I resulted in changes in the conformation of the adsorbed molecule. These changes in conformation altered the binding of specific integrins (α3β1) to fibronectin. This modulation in integrin binding controlled osteoblast-specific gene expression in human SaOs-2 osteoblast-like cells. The principle of controlling cell function by conformational changes in the extracellular matrix represents a powerful approach to engineer surfaces that elicit targeted cellular responses
Keywords :
bone; cellular biophysics; genetics; molecular biophysics; molecular configurations; proteins; adsorbed molecule conformation; cell function control; collagen type I; fibronectin conformation; human SaOs-2 osteoblast-like cells; osteoblast-specific gene expression; osteoblastic phenotype modulation; specific integrins binding; substrate-dependent changes; surface engineering; targeted cellular responses; tissue culture grade polystyrenes; Adhesives; Biomedical engineering; Cells (biology); Extracellular; Gene expression; Humans; Mechanical engineering; Mineralization; Power engineering and energy; RNA;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.802151
