Title :
Tissue-engineered microvascular networks for gene therapy
Author :
Gooch, K.J. ; Sieminski, A.L. ; Padera, R.F. ; Blunk, T. ; Mulligan, R.C. ; Langer, R.
Author_Institution :
Dept. of Bioeng., Pennsylvania Univ., Philadelphia, PA, USA
Abstract :
The authors have explored the use of tissue-engineered microvascular networks for gene therapy. Rat microvascular endothelial cells (RMEC), engineered to secrete human growth hormone (hGH), continued to secrete hGH after forming microvascular networks in Matrigel. When hGH-releasing microvascular networks were implanted subcutaneously into nude mice the hGH concentration in serum, while decreasing initially, leveled off at ~0.2 ng/ml and remained stable for over ten months
Keywords :
blood vessels; genetics; patient treatment; 10 month; Matrigel; gene therapy; human growth hormone secretion; nude mice; rat microvascular endothelial cells; serum; subcutaneous implantation; tissue-engineered microvascular networks; Biomedical engineering; Blood; Cells (biology); Embryo; Gene therapy; Implants; In vitro; In vivo; Mice; Proteins;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.802164
