Title :
Optical mapping of anatomical reentry in monolayers of cultured neonatal rat cardiac myocytes
Author :
Lu, Steven N. ; Entcheva, Emilia ; Sharma, Vinod ; Tung, Leslie
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Abstract :
There were two primary objectives in the study. First, we present a novel macroscopic optical method with high sensitivity to record transmembrane potentials, Vm, in monolayers of cultured cardiac myocytes. Second, using this technique we demonstrate, for the first time, electrically-induced, sustained reentrant activity in a two-dimensional (2-D) cell culture preparation
Keywords :
bioelectric potentials; biological techniques; biomembrane transport; cardiology; fibre optic sensors; monolayers; muscle; anatomical reentry; cultured neonatal rat cardiac myocytes; electrically-induced sustained reentrant activity; macroscopic optical method; monolayers; optical mapping; transmembrane potentials; two-dimensional cell culture preparation; Biomedical optical imaging; Fluorescence; Heart; Optical fibers; Optical filters; Optical recording; Optical sensors; Pediatrics; Spatial resolution; Voltage;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.802181
