A confocal setup to measure transmembrane potential in cardiac tissue with very high spatial and temporal resolution

Author

Dapra, D. ; Muenzer, T. ; Ahammer, H. ; Guenter, C.I. ; Lackner, M. ; Windisch, H.

Author_Institution

Inst. for Med. Phys. & Biophys., Graz Univ., Austria

Volume

1

fYear

1999

fDate

1999

Abstract

We used a fast, fluorescent, potential sensitive indicator (di-4-ANEPPS) in combination with a specially designed setup which consists of an fluorescence microscope, a frequency doubled Nd:YAG laser and a 256 photodiode array system. The setup is especially designed to investigate tissue preparations in the mm range. The common setup can be reconstructed easily to a confocal laser microscope. This allows confocal microscopic detection of transmembrane potential with high temporal and spatial resolution. The axial resolution of the confocal results in a FWHM better then 15 μm. Signals from papillary muscle were compared to images recorded by a CLSM (Leica TCS NT)

Keywords

bio-optics; bioelectric potentials; biomedical equipment; biomedical imaging; biomedical measurement; biomembrane transport; cardiology; fluorescence; laser applications in medicine; muscle; optical microscopes; optical microscopy; YAG:Nd; YAl5O12:Nd; axial resolution; cardiac tissue; confocal laser microscope; confocal setup; fast indicator; fluorescence microscope; fluorescent potential sensitive indicator; frequency doubled Nd:YAG laser; high spatial resolution; high temporal resolution; papillary muscle; photodiode array system; transmembrane potential measurement; Fluorescence; Frequency; Image reconstruction; Microscopy; Muscles; Optical arrays; Optical design; Photodiodes; Signal resolution; Spatial resolution;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802209

Filename

802209