Miniaturized portable image-recording device for paper-based nucleotide assays with nanoparticles

Author

Lo, S.J. ; Wan, D.H. ; Cheng, C.M. ; Keranen, K. ; Korhonen, R. ; Kopola, H. ; Yao, D.J.

Author_Institution

Inst. of Nanoengineering & Microsyst., Nat. Tsing Hua Univ., Hsinchu, Taiwan

fYear

2015

fDate

21-25 June 2015

Firstpage

1735

Lastpage

1738

Abstract

This study describes the development of a portable image-recording device that is lightweight, inexpensive, and compatible with commercial smartphones and paper-based analytical devices. A light-unaffected environment is essential for optical quantification in colorimetric-based or fluorescence-based assays, and we have previously developed a portable fluorescent image-recording device. Here, we attempted to miniaturize the portable image-recording device by using a thin-film LED strip and minimize the cost of the device by using colored glassine as a light filter. Finally, we demonstrated the capacity to measure DNA-AuNPs concentration with a paper-based device.

Keywords

DNA; biomedical equipment; biomedical optical imaging; chemical sensors; colorimetry; fluorescence spectroscopy; gold; light emitting diodes; molecular biophysics; nanomedicine; nanoparticles; paper technology; portable instruments; smart phones; spectrochemical analysis; thin films; Au; DNA-AuNP concentration measurement; colored glassine; colorimetric-based assay; commercial smartphone compatibility; device cost minimization; fluorescence-based assay; light filter; light-unaffected environment; miniaturized portable image-recording device; nanoparticle; optical quantification; paper-based analytical device; paper-based nucleotide assay; portable fluorescent image-recording device; portable image-recording device development; thin-film LED strip; Cameras; DNA; Image color analysis; Light emitting diodes; Nanoparticles; Smart phones; Software; Light-emitting diode; Nanoparticle; Paper-based device;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181280

Filename

7181280