Title :
Computational model of DIC microscopy for reconstructing specimens
Author :
Kagalwala, F. ; Lanni, F. ; Kanade, T.
Author_Institution :
Robotics Inst., Carnegie Mellon Univ., Pittsburgh, PA, USA
Abstract :
Biologists often use differential interference contrast (DIC) microscopy to study live cells. However, they are limited to qualitative observations due to the inherent nonlinear relation between the object properties and image intensity. As a first step towards quantitatively measuring optical properties of objects from DIC images, we develop and verify a computational model for the image formation process. Next, we plan to use this model to reconstruct the properties of unknown specimens
Keywords :
biological techniques; cellular biophysics; image reconstruction; light interference; modelling; optical microscopy; ray tracing; computational model; differential interference contrast microscopy; image formation process; image intensity; inherent nonlinear relation; live cells; object properties; optical properties; unknown specimens; Biological system modeling; Biology computing; Biomedical optical imaging; Cells (biology); Computational modeling; Image reconstruction; Interference; Microscopy; Nonlinear optics; Optical computing;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.803978
