Autoradiography of high energy radionuclides using a microchannel plate detector

Author

Lees, J.E. ; Murray, A. ; Perkins, A.C. ; Fraser, G.W.

Author_Institution

BioImaging Unit, Leicester Univ., UK

Volume

3

fYear

2000

fDate

2000

Abstract

Reports on the use of a microchannel plate-based imaging system for autoradiography of biological samples labeled with radionuclides emitting high-energy (140-2000 keV) beta particle and gamma radiation. Samples can be imaged sufficiently rapidly (exposures 1-2 hours for electrophoresis gels, ~24 hours for thin tissue sections) to allow imaging of nuclides with short half-lives, such as ^99mTc. Measurement of the detector efficiencies for ^99mTc and ¹⁸⁸Re isotopes is reported. Images of a variety of samples labeled with clinically important therapeutic radionuclides, ⁶⁷Cu and ¹³¹I are presented. In particular the authors report preliminary images of radiolabeled anti-MUCI mucin antibody C595 currently being developed as a therapeutic conjugate for treating bladder, breast and ovarian tumours

Keywords

beta-ray detection; biological techniques; gamma-ray detection; microchannel plates; radiation therapy; radioisotope imaging; 1 to 2 h; 140 to 2000 keV; 24 h; ¹³¹I; ¹⁸⁸Re isotopes; ⁶⁷Cu; ^99mTc; Cu; I; Re; Tc; autoradiography; bladder; breast; clinically important therapeutic radionuclides; electrophoresis gels; high energy radionuclides; labeled biological samples; microchannel plate detector; ovarian tumours; therapeutic conjugate; thin tissue sections; Beta rays; Bladder; Breast; Detectors; Electrokinetics; Gamma rays; Isotopes; Microchannel; Optical imaging; Tumors;

fLanguage

English

Publisher

ieee

Conference_Titel

Nuclear Science Symposium Conference Record, 2000 IEEE

Conference_Location

Lyon

ISSN

1082-3654

Print_ISBN

0-7803-6503-8

Type

conf

DOI

10.1109/NSSMIC.2000.949364

Filename

949364