Title :
A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries
Author :
Kim, Duwoon ; Kim, Seok-Ryel ; Day, Donal F. ; Oh, Myung-Joo
Author_Institution :
Div. of Food Sci. & Aqualife Med., Chonnam Nat. Univ., Gwangju
Abstract :
Sephadextrade bead agarose electrophoresis (SBAE) was used to enrich the higher dextran binding phages. Phage collection obtained after the 2nd round of SBAE was 3.5 fold higher color intensity than that of phage collections obtained after the 1st round. DNA sequencing of phage collection (SBAE-2R) confirmed that human origin antibody was present. The PCR products of lambda, k light chains and heavy chain from phage collection (SBAE-2R) were approximately 420 bp, 550 bp and 600 bp.
Keywords :
DNA; electrophoresis; DNA sequencing; Sephadex bead agarose electrophoresis; color intensity; dextran binding antibody; human origin antibody; phage display libraries; DNA; Displays; Electrokinetics; Humans; Immune system; Libraries; Milling machines; Proteins; Sugar industry; Sugar refining;
Conference_Titel :
Frontiers in the Convergence of Bioscience and Information Technologies, 2007. FBIT 2007
Conference_Location :
Jeju City
Print_ISBN :
978-0-7695-2999-8
DOI :
10.1109/FBIT.2007.73
