• DocumentCode
    3477440
  • Title

    A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries

  • Author

    Kim, Duwoon ; Kim, Seok-Ryel ; Day, Donal F. ; Oh, Myung-Joo

  • Author_Institution
    Div. of Food Sci. & Aqualife Med., Chonnam Nat. Univ., Gwangju
  • fYear
    2007
  • fDate
    11-13 Oct. 2007
  • Firstpage
    64
  • Lastpage
    68
  • Abstract
    Sephadextrade bead agarose electrophoresis (SBAE) was used to enrich the higher dextran binding phages. Phage collection obtained after the 2nd round of SBAE was 3.5 fold higher color intensity than that of phage collections obtained after the 1st round. DNA sequencing of phage collection (SBAE-2R) confirmed that human origin antibody was present. The PCR products of lambda, k light chains and heavy chain from phage collection (SBAE-2R) were approximately 420 bp, 550 bp and 600 bp.
  • Keywords
    DNA; electrophoresis; DNA sequencing; Sephadex bead agarose electrophoresis; color intensity; dextran binding antibody; human origin antibody; phage display libraries; DNA; Displays; Electrokinetics; Humans; Immune system; Libraries; Milling machines; Proteins; Sugar industry; Sugar refining;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Frontiers in the Convergence of Bioscience and Information Technologies, 2007. FBIT 2007
  • Conference_Location
    Jeju City
  • Print_ISBN
    978-0-7695-2999-8
  • Type

    conf

  • DOI
    10.1109/FBIT.2007.73
  • Filename
    4524081
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=3477440