Title :
Principal Component Analysis of Infectomes in Human Brain Endothelial Cell after C. neoformans Infection
Author :
Jong, Ambrose Y. ; Feng, Luo ; Chen, Steven H M ; Huang, Sheng-He
Author_Institution :
Div. of Hematology-Oncology, Children´´s Hosp. Los Angeles, Los Angeles, CA
Abstract :
Cell surface molecule CD44 of human brain microvascular endothelial cells (HBMEC) is the receptor of Cryptococcus neoformans during the infection. We performed microarray analysis and used principle component analysis to examine gene profiles of HBMEC in responses to C neoformans infection. The CD44 levels increases, peaking at the 4-hour time point and then declined gradually. Seventy genes displayed a similar gene profile. Our results showed a dynamic relationship between pathogen-host interactions. The data is useful for the analysis of invasion mechanism of C neoformans into HBMEC.
Keywords :
brain; cellular biophysics; diseases; genetics; microorganisms; molecular biophysics; neurophysiology; principal component analysis; C. neoformans infection; Cryptococcus neoformans; cell surface molecule; endothelial cell; gene profiles; genes; human brain cell; infectomes; microarray analysis; microvascular cells; pathogen-host interactions; principal component analysis; time 4 hour; Cryptography; Gene expression; Hospitals; Humans; Information technology; Multidimensional systems; Pathogens; Performance analysis; Principal component analysis; Temperature sensors;
Conference_Titel :
Frontiers in the Convergence of Bioscience and Information Technologies, 2007. FBIT 2007
Conference_Location :
Jeju City
Print_ISBN :
978-0-7695-2999-8
DOI :
10.1109/FBIT.2007.134
