DocumentCode :
3491243
Title :
Analysis of gene expression profile triggered by signal peptide of eosinophil cationic protein
Author :
Liu, Yu-Shu ; Chao, Chung-Hsaio ; Chang, Hao-Teng ; Chang, Margaret ; Wang, Yong ; Pai, Tun-Wen
Author_Institution :
Grad. Inst. of Mol. Syst. Biomed., China Med. Univ., Taichung, Taiwan
fYear :
2011
fDate :
2-4 Sept. 2011
Firstpage :
137
Lastpage :
143
Abstract :
The signal peptide of eosinophil cationic protein (ECPsp) is known to play an important role in translocating ECP to extracellular space. However, we previously discovered that ECPsp has a novel function of inhibiting microbial growth and regulating the gene expression of tumor growth factor-alpha (TGF-α) and epidermal growth factor receptor (EGFR) in mammalian cells. In the present study, we first generated a DNA microarray dataset, which showed that ECPsp up-regulated inflammatory molecules including cytokines, chemokines, interferon-induced molecules, and Toll-like receptors. We then generated a function linkage network by integrating the microarray dataset with the KEGG pathway database, and discovered that STAT1, an important factor regulating cytokine expression and release, served as a hub to connect the pathways of cytokine stimulation (TGF-α and EGFR) and inflammatory responses. Furthermore, integrating the ECPsp interactome dataset with the functional linkage network elucidated that STAT1 served as a hub to connect 3 functional clusters, including cell proliferation and survival, protein translational regulation, and inflammatory responses. Our approach involving experimental and computational systems biology provided predicted pathways and potential regulation for further characterization of the novel function of ECPsp under inflammatory conditions.
Keywords :
DNA; biology computing; blood; cancer; cellular transport; genetics; molecular biophysics; proteins; tumours; DNA microarray dataset; KEGG pathway database; STAT1; Toll-like receptors; cell proliferation; cell survival; chemokines; computational systems biology; cytokines; eosinophil cationic protein; epidermal growth factor receptor; gene expression profile; inflammatory molecule; interferon induced molecules; mammalian cell; microbial growth; protein translational regulation; signal peptide; tumor growth factor alpha; Couplings; DNA; Gene expression; Humans; Peptides; Proteins; Systems biology; eosinophil cationic protein; functional linkage network; gene regulation; inflammation; signal peptide;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Systems Biology (ISB), 2011 IEEE International Conference on
Conference_Location :
Zhuhai
Print_ISBN :
978-1-4577-1661-4
Electronic_ISBN :
978-1-4577-1665-2
Type :
conf
DOI :
10.1109/ISB.2011.6033145
Filename :
6033145
Link To Document :
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