Title :
Magnetophoresis for mononuclear cell separation and diagnostic evaluation of the phagocytic capacity of monocyte
Author :
Minamitani, Haruyuki ; Shimomura, Takuji ; Aiba, Masayuki ; Okada, Eiji
Author_Institution :
Dept. of Electr. Eng., Keio Univ., Yokohama, Japan
Abstract :
The authors discuss magnetophoresis, which specifically separates monocytes phagocytizing iron dextran microspheres from mononuclear cell suspensions under a DC magnetic field. Diagnostic evaluation of the phagocytic capacity was carried out for patients with liver diseases on the basis of the separation efficiency of the monocytes. Results of the magnetic separation showed that the phagocytic capacities of hepatoma and liver cirrhosis patients were significantly lower than those of healthy volunteers. Magnetophoresis was found to be essential in investigating the phagocytosis.<>
Keywords :
biomagnetism; cellular biophysics; liver; patient diagnosis; Fe dextran microspheres; hepatoma; liver cirrhosis; liver disease patients; magnetophoresis; mononuclear cell separation; phagocytic capacity evaluation;
Conference_Titel :
Engineering in Medicine and Biology Society, 1988. Proceedings of the Annual International Conference of the IEEE
Conference_Location :
New Orleans, LA, USA
Print_ISBN :
0-7803-0785-2
DOI :
10.1109/IEMBS.1988.95127
