Title :
Fabrication of micro pore arrays in free standing COC membranes and their application for in vitro barrier tissue models
Author :
Gel, M. ; Kandasamy, Sivasathivel ; Cartledge, K. ; Haylock, D.
Author_Institution :
Mater. Sci. & Eng., CSIRO, Clayton, VIC, Australia
Abstract :
This paper reports a low-cost fabrication method for mass production of free standing cyclic olefin copolymer (COC) membranes embedded with micro pore arrays. We show that the method creates suspended membranes with thickness of 2μm and micro pores having diameter ranging from 3-5μm. We integrated the membrane in a microfluidic system designed to mimic the endothelial barrier in bone marrow sinusoids. The device was tested with HUVEC and supported their attachment and proliferation on the membrane.
Keywords :
bioMEMS; biological tissues; biomedical materials; biomembranes; bone; cellular biophysics; microfluidics; molecular biophysics; polymer blends; porosity; tissue engineering; HUVEC; bone marrow sinusoids; endothelial barrier; free standing cyclic olefin copolymer membranes; in vitro barrier tissue models; low-cost fabrication method; mass production; membrane attachment; membrane proliferation; microfluidic system; micropore array fabrication; size 2 mum; size 3 mum to 5 mum; suspended membranes; Biomembranes; Bones; Cells (biology); Fabrication; Films; In vitro; Microfluidics;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
Conference_Location :
Taipei
Print_ISBN :
978-1-4673-5654-1
DOI :
10.1109/MEMSYS.2013.6474388
