Cell Fatigue Test

Author

Fukui, Wataru ; Kaneko, Makoto ; Sakuma, Shinya ; Arai, Fumihito

Author_Institution

Dept. of Mech. Eng., Osaka Univ., Suita, Japan

fYear

2013

fDate

20-24 Jan. 2013

Firstpage

1025

Lastpage

1028

Abstract

A new concept of “Cell Fatigue Test” is proposed. By reciprocating a cell across the throat of a micro channel repeatedly, the dynamic behavior of cell deformation is measured. We define a new index of fatigue life of cells as the number of reciprocatory motions leading to the prescribed recovery ratio. The test system is composed of a piezoelectric (PZT) actuator, a high speed vision sensor and a micro channel with a throat. Preliminary experiments were conducted by using Red Blood Cells (RBCs). The result shows that there is great variability in fatigue life of cells and the hues of cell membrane drastically change as the number of stress cycles increase.

Keywords

bioMEMS; biomechanics; biomembranes; blood; cellular biophysics; deformation; fatigue; image sensors; piezoelectric actuators; cell deformation measurement; cell fatigue test; cell membrane; dynamic behavior; high speed vision sensor; microchannel; piezoelectric actuator; reciprocatory motions; red blood cells; stress cycles; Actuators; Diseases; Fatigue; Indexes; Microscopy; Sensors; Stress;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on

Conference_Location

Taipei

ISSN

1084-6999

Print_ISBN

978-1-4673-5654-1

Type

conf

DOI

10.1109/MEMSYS.2013.6474423

Filename

6474423