Title :
An innovative sample-to-answer polymer lab-on-a-chip with on-chip reservoirs for the POCT of thyroid stimulating hormone (TSH)
Author :
Jung, Wanyeong ; Han, Jinguang ; Kai, J. ; Lim, Jungyoul ; Sul, D. ; Ahn, Chong H.
Author_Institution :
Microsyst. & BioMEMS Lab., Univ. of Cincinnati, Cincinnati, OH, USA
Abstract :
A new sample-to-answer polymer lab-on-a-chip, which can perform immunoassay with minimum user intervention through on-chip reservoirs for reagents and single microchannel assay, has been designed, developed and successfully characterized as a point-of-care testing (POCT) cartridge for the detection of thyroid stimulating hormone (TSH). Users only need to drop a sample to the POCT cartridge to obtain test results within 30 minutes. The analyzed results of TSH showed a linear range up to 55 μIU/mL with the limit of detection (LOD) of 1.9 μIU/mL at the signal to noise ratio (SNR) of 3. The reagents stored in the on-chip reservoirs maintained their volume more than 97 % of initial volume for 100 days of storage time while the detection antibody retained its activity above 97 %.
Keywords :
bioMEMS; biological organs; biomedical measurement; lab-on-a-chip; patient diagnosis; polymers; antibody detection; innovative sample-to-answer polymer lab-on-a-chip; minimum user intervention; on-chip reservoirs; point-of-care testing cartridge; reagent storing; signal-to-noise ratio; single microchannel assay; storage time; thyroid stimulating hormone; time 100 d; time 30 min; Computer numerical control; Educational institutions; Lab-on-a-chip; Polymers; Protocols; Spirals; Substrates;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
Conference_Location :
Taipei
Print_ISBN :
978-1-4673-5654-1
DOI :
10.1109/MEMSYS.2013.6474430
