Title :
All-in-one droplet platform for multiplexed genetic detection in blood
Author :
Yi Zhang ; Tza-Huei Wang
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Abstract :
Presents a fully integrated magnetic droplet platform capable of performing all steps from sample preparation to target analysis for multiplexed genetic biomarker detection with a single device. On this platform, surface energy traps (SETs) are implemented to enable complex liquid handling including droplet moving, merging, immobilization, dispensing, metering and aliquoting, which greatly enhances the functionality of a microfluidic droplet chip to carry out steps for cell lysis, solid phase DNA extraction, DNA aliquoting, DNA-PCR primer mixing, and emulsion PCRs in droplets.
Keywords :
DNA; bioMEMS; biochemistry; biological specimen preparation; biomagnetism; blood; cellular biophysics; drops; emulsions; enzymes; genetics; genomics; lab-on-a-chip; magnetic fluids; microfluidics; mixing; molecular biophysics; surface energy; DNA aliquoting; DNA-PCR primer mixing; all-in-one droplet platform; blood; cell lysis; complex liquid handling; droplet aliquoting; droplet dispensing; droplet immobilization; droplet merging; droplet metering; droplet moving; emulsion polymerase chain reaction; fully integrated magnetic droplet platform; microfluidic droplet chip; multiplexed genetic biomarker detection; sample preparation; solid phase DNA extraction; surface energy traps; target analysis; Blood; Cancer; DNA; Fluorescence; Multiplexing; Plugs; Surface topography;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
Conference_Location :
Taipei
Print_ISBN :
978-1-4673-5654-1
DOI :
10.1109/MEMSYS.2013.6474432
