All-in-one droplet platform for multiplexed genetic detection in blood

Author

Yi Zhang ; Tza-Huei Wang

Author_Institution

Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA

fYear

2013

fDate

20-24 Jan. 2013

Firstpage

1061

Lastpage

1064

Abstract

Presents a fully integrated magnetic droplet platform capable of performing all steps from sample preparation to target analysis for multiplexed genetic biomarker detection with a single device. On this platform, surface energy traps (SETs) are implemented to enable complex liquid handling including droplet moving, merging, immobilization, dispensing, metering and aliquoting, which greatly enhances the functionality of a microfluidic droplet chip to carry out steps for cell lysis, solid phase DNA extraction, DNA aliquoting, DNA-PCR primer mixing, and emulsion PCRs in droplets.

Keywords

DNA; bioMEMS; biochemistry; biological specimen preparation; biomagnetism; blood; cellular biophysics; drops; emulsions; enzymes; genetics; genomics; lab-on-a-chip; magnetic fluids; microfluidics; mixing; molecular biophysics; surface energy; DNA aliquoting; DNA-PCR primer mixing; all-in-one droplet platform; blood; cell lysis; complex liquid handling; droplet aliquoting; droplet dispensing; droplet immobilization; droplet merging; droplet metering; droplet moving; emulsion polymerase chain reaction; fully integrated magnetic droplet platform; microfluidic droplet chip; multiplexed genetic biomarker detection; sample preparation; solid phase DNA extraction; surface energy traps; target analysis; Blood; Cancer; DNA; Fluorescence; Multiplexing; Plugs; Surface topography;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on

Conference_Location

Taipei

ISSN

1084-6999

Print_ISBN

978-1-4673-5654-1

Type

conf

DOI

10.1109/MEMSYS.2013.6474432

Filename

6474432