Title :
Two-photon imaging with 15-fs pulses and third-harmonic microscopy imaging techniques
Author :
Squier, Jeffrey A. ; Fittinghoff, D.N. ; Barty, C.P.J. ; Wilson, K.R. ; Muller, Mathias ; Buist, A.H. ; Brakenhoff, G.J.
Author_Institution :
Dept. of Electr. & Comput. Eng., California Univ., San Diego, La Jolla, CA, USA
Abstract :
Summary form only given. We have shown that it is possible to produce very short pulses at the focus of a high-NA system. These pulses have been used to produce real-time two-photon images, without the aid of an additional amplifier. The third harmonic is shown to be a useful tool for characterizing the pulse within these systems and to be of sufficient strength that it can be used as an imaging tool as well. The characteristics of the third-harmonic imaging are undergoing further investigation so that this method can be exploited as efficiently as possible.
Keywords :
high-speed optical techniques; optical focusing; optical harmonic generation; optical microscopy; two-photon processes; fs pulses; high-NA system focus; imaging tool; real-time two-photon images; third harmonic; third-harmonic imaging; third-harmonic microscopy imaging techniques; two-photon imaging; very short pulses; Diffraction gratings; Holographic optical components; Liquid crystal polymers; Liquid crystals; Microscopy; Optical filters; Optical imaging; Optimized production technology; Photorefractive effect; Photorefractive materials;
Conference_Titel :
Lasers and Electro-Optics, 1998. CLEO 98. Technical Digest. Summaries of papers presented at the Conference on
Conference_Location :
San Francisco, CA, USA
Print_ISBN :
1-55752-339-0
DOI :
10.1109/CLEO.1998.675825