Stochastic 3-D signal reconstruction from noisy projection data for heterogeneous instances of objects in electron microscopy imagery

Author

Zheng, Yili ; Wang, Qiu ; Doerschuk, Peter C.

Author_Institution

Comput. Res. Div., Lawrence Berkeley Nat. Lab., Berkeley, CA, USA

fYear

2011

fDate

March 30 2011-April 2 2011

Firstpage

918

Lastpage

921

Abstract

Different instances of a biological macromolecular complex need not share the same 3-D electron scattering distribution function due to stoichiometric variability, flexibility and vibrations, etc. Cryo electron microscopy provides 2-D images of each of many such complexes where each image is roughly a projection. A statistical estimation problem is described and demonstrated for determining a statistical description of the complex from such imagery.

Keywords

biological techniques; biology computing; biothermics; electron microscopy; macromolecules; molecular biophysics; molecular configurations; signal processing; stochastic processes; 3D electron scattering distribution function; biological macromolecular complex; cryoelectron microscopy; electron microscopy imagery; heterogeneous object instances; noisy projection data; statistical estimation problem; stochastic 3D signal reconstruction; Biology; Covariance matrix; Electron microscopy; Image reconstruction; Maximum likelihood estimation; USA Councils; 3-D image reconstruction; cryo electron microscopy; maximum likelihood estimation; tomography; virus structure;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: From Nano to Macro, 2011 IEEE International Symposium on

Conference_Location

Chicago, IL

ISSN

1945-7928

Print_ISBN

978-1-4244-4127-3

Electronic_ISBN

1945-7928

Type

conf

DOI

10.1109/ISBI.2011.5872553

Filename

5872553