Immobilization of biological macromolecule on titanium oxide film to improve the biocompatibility

Summary form only given. Ti-O film was prepared by magnetron sputtering. The surface of Ti-O film was pretreated with H/sub 2/O/sub 2/ and HCl mixed solution to get a surface with -OH group. The aminopropyltriethoxysilane (APTE) was covalently interacted with -OH group on the surface of Ti-O film. Then biological macromolecule such as albumin and heparin was immobilized through covalent chemical attachment between the -NH/sub 2/ group of the APTE and the -OH group of Ti-O film. X-ray photoelectron spectroscopy (XPS), Atomic Force Microscopy (AFM), and Fourier transform infrared spectroscopy (FTIR) was used to investigate the characteristic of the film of APTE, albumin and heparin on the surface of Ti-O film. The number and the morphology of blood platelet were studied by blood platelet adhesion test in vitro. Endothelial cell culture test was used to investigate the function and influence between materials and the endothelial cells. The results showed that the surface of Ti-O film could be coated covalently with the albumin and heparin. Blood platelet adhesion test proved that the number of blood platelet decreased 50%, meanwhile the morphology of blood platelet was better than the untreated Ti-O film surface. Endothelial cell test showed that the surface coated with albumin and heparin influenced the number and status of endothelial cells compared with the untreated Ti-O film surface. This investigation showed that inorganic surface immobilized with biologic molecular is feasible and effective to improve the biocompatibility of bio-inert materials.