Lactobacillus delbrueckii mediated synthesis of silver nanoparticles and their evaluation of antibacterial efficacy against MDR clinical pathogens

The development of reliable and eco-friendly biosynthetic process of silver nanoparticles acts as a vital role in the field of nano-biotechnology today. The present investigation reported that extracellular rapid biosynthesis of silver nanoparticles (Ag-NPs) using Lactobacillus delbrueckii isolated from probiotic curd. The characterization of nanoparticles were carried out by UV-visible spectrophotometer, Fourier Transform Infrared Spectroscopy (FTIR), Thin Layer Chromatography (TLC), X-ray diffraction Analysis (XRD) and Scanning Electron Microscopy (SEM). FTIR characterization was made for the analysis of reducing protein used for the synthesis of silver nanoparticles. SEM analysis was confirmed that nanoparticles size in range of 54.3 nm to 112.7 nm. The present analysis authenticate that biologically synthesized silver nanoparticles played a potential role in significant higher antimicrobial efficacy against multi drug resistant Klebsiella pneumonia and Staphylococcus aureus.