Title :
Real-time monitoring of photothermal porated mammalian cells by electric impedance sensors
Author :
Yamane, Daisuke ; Wu, Yi-Chien ; Wu, Ting-Hsiang ; Toshiyoshi, Hiroshi ; Teitell, Michael A. ; Chiou, Pei-Yu
Author_Institution :
Dept. of Mech. & Aerosp. Eng., Univ. of California, Los Angeles, Los Angeles, CA, USA
Abstract :
We present a novel electrical impedance sensor for measuring the resealing time of porated mammalian plasma membranes. Openings were generated by high speed cavitation bubbles using laser pulsing of metallic microdisks on transparent ITO electrodes. Real-time impedance measurements show that membrane resealing time and impedance recovery takes 1 to 2 minutes after laser pulsing. Our method can also detect cells under high energy laser pulsing that experience immediate death and a one-way impedance shift.
Keywords :
biomembrane transport; bubbles; cavitation; cellular biophysics; electric impedance measurement; electrodes; indium compounds; laser applications in medicine; photothermal effects; InSnO; cavitation bubbles; electric impedance sensors; electrodes; impedance recovery; laser pulsing; membrane resealing time; metallic microdisks; one way impedance shift; photothermal porated mammalian cells; porated mammalian plasma membranes; real time impedance measurements; real time monitoring; Biomembranes; Cells (biology); Impedance; Impedance measurement; Lasers; Measurement by laser beam; Sensors; cell membrane; cell surgery; impedance sensor; membrane pores; photothermal effect; pulsed laser;
Conference_Titel :
Optical MEMS and Nanophotonics (OMN), 2012 International Conference on
Conference_Location :
Banff, AB
Print_ISBN :
978-1-4577-1511-2
DOI :
10.1109/OMEMS.2012.6318882
