Effective low drug release using gold nanoparticles from chryseobacterium Spp against oral pathogens

Dental care is an important phenomenon in human health; it involves number of pathogenic microbial population which in turns involves in softening of teeth, improper cavity formation and color changes in teeth. Oral pathogens can cause severe damage which may lead to serious issues in human disease like coronary disease and blood circulation. Conventional treatments of oral pathogens using antibiotics were effective in particular proportion of time period. Implementation of nanotechnology is effective tool for slow releasing of antibiotic in controlled way against oral pathogens like Candida spp., Streptococcus spp., Lactobacillus spp., etc. Gold nano particles were tried both chemical method as well as biological method. Trisodium Citrate act as reducing agent to produce gold nano colloid particle in chemical method. Chryseobacterium Spp has the ability to produce secondary metabolites which has the reduction ability of gold particles. It was confirmed by the peak observed in UV spectrophotometer at 525nm. It may involve the enzymes like reductase and dehydrogenase. Chryseobacterium Spp has the reducing ability of gold nanoparticles in time period of 4 days. It has the particle diameter of 100-250nm in SEM analysis. Implementation of the synthesized nanoparticles for drug release is a preliminary mechanism of Drug delivery in a particular target receptor. Antibiotics like gentamycin, ceftaximide were used in 1:10 ratio with gold nano particles using Ultrasonicator for 12hrs. Then the mixtures were prepared different concentration (20μg/ml, 40 μg/ml, 60 μg/ml etc.,) for well diffusion techniques to measure the release of antibiotic in controlled process. Here E.Coli, Pseudomonas fiuorecense, Candida albicans, Candida tropicans and Candida trousi plates were prepared and measured the time and zone of inhibition. Zone of inhibition of combined gold nanoparticles with antibiotic showed the diameter of 45mm in first day compared to that of antib- otic alone as 40mm. In second and third day of diameter gets increased 47mm, and 50mm respectively. But in case of control it was at constant as 40mm against oral pathogen. It indicates the combined effect of nanoparticles and antibiotic has the slow release of drug molecule against oral pathogens.