Title :
Volume microscopy of biological specimens based on non-confocal imaging techniques
Author :
Senft, Stephen L. ; Argiro, Vincent J. ; VanZandt, William L.
Author_Institution :
Washington Univ. Sch. of Med., St. Louis, MO, USA
Abstract :
An approach that uses advanced computer graphics workstations and volume rendering algorithms for accurate reconstruction of volumetric microscopy data is described. It has been found that excellent reconstructions can be made from serial sections acquired using a charge-coupled device and a conventional light microscope. Both confocal and nonconfocal reconstructions are examined. The effects of differing light sources are considered 3D image processing results are presented
Keywords :
biological techniques and instruments; computer graphics; computerised instrumentation; computerised picture processing; optical microscopy; 3D image processing; advanced computer graphics workstations; biological specimens; charge-coupled device; confocal reconstruction; light microscope; light sources; nonconfocal reconstructions; reconstruction; serial sections; volume rendering algorithms; volumetric microscopy data; Biomedical optical imaging; Computer graphics; Data visualization; Geometrical optics; Image reconstruction; Nervous system; Optical imaging; Optical microscopy; Rendering (computer graphics); Workstations;
Conference_Titel :
Visualization, 1990. Visualization '90., Proceedings of the First IEEE Conference on
Conference_Location :
San Francisco, CA
Print_ISBN :
0-8186-2083-8
DOI :
10.1109/VISUAL.1990.146413
