Title :
Effect of flow on prostaglandin E2 production and inositol trisphosphate levels in cultured osteoblasts
Author :
Reich, Kathleen ; Gay, Carol ; Frangos, John
Author_Institution :
Dept. of Chem. Eng., Pennsylvania State Univ., University Park, PA, USA
Abstract :
In order to determine what effects flow has on prostaglandin production, prostaglandin E2 levels were analyzed in perfusing media. To examine the effect of flow on cultured osteoblasts, intracellular concentrations of inositol trisphosphate were measured. It is shown that prostaglandin E2 production is increased in cells exposed to either steady or pulsatile flow. Flow-caused oscillations were observed in the intracellular levels of inositol trisphosphate. These responses are both shear and time dependent
Keywords :
biorheology; bone; cellular biophysics; cultured osteoblasts; flow caused oscillations; inositol trisphosphate; intracellular concentrations; monolayered cultures; perfusing media; prostaglandin E2 levels; prostaglandin E2 production; pulsatile flow; shear dependent; time dependent; Biochemistry; Bones; Cells (biology); DNA; Humans; Joints; Production; Stress;
Conference_Titel :
Bioengineering Conference, 1990., Proceedings of the 1990 Sixteenth Annual Northeast
Conference_Location :
State College, PA
DOI :
10.1109/NEBC.1990.66329
