NTP-mediated changes of gene expression patterns in human cell lines

In the field of wound healing research non-thermal plasma (NTP) has attracted increasing attention over the last decades. NTP has a complex composition and consists of ions, exited atoms, electrons, ultraviolet (UV) light, visible and infrared radiation, neutral molecules, and free radicals e.g. reactive oxygen and nitrogen species (ROS/RNS)¹. Next to its well-established antibacterial effects it has potential proliferation promoting properties on eukaryotic cells². Although a number of studies have been carried out to investigate the impact of NTP on human cells, not much is known about the influences on the molecular level. In order to shed some light in the complex impact NTP has on wound healing, this study analyzed in detail the differential gene expression pattern of human cells involved in this process in response to NTP treatment. Besides the keratinocyte cell line HaCaT, two immune cells lines were studied - the CD4⁺ T helper cell line Jurkat and the monocyte cell line THP-1.