Treatment of oral pathogenic bacteria with non-thermal plasma activated water as a new type mouthwash

Summary form only given. Dental caries and periodontal disease are common oral diseases worldwide, and 60-90% of school children and nearly 100% of adults have dental cavities. The world health organization has put the incidence of periodontal disease as the tumor, cardiovascular morbidity after the third. Actually, those two diseases are caused by bacteria (facultative anaerobes and obligate anaerobes). Chemical drug (such as 0.2% chlorhexidine) is one of the primary clinical prevention and treatment for common oral diseases in clinical practice. However, chlorhexidine often leads to inevitable side effects, such as: tooth staining, taste disturbance, mucosal erosion, unilateral or bilateral swelling of the parotid gland, etc.. In our study, Ar/O2 (2%) non-thermal plasma micro jet (PMJ) was used to treat distilled water for 20 min to create plasma activated water (PAW), then the produced PAW was applied to three oral pathogens: Streptococcus mutans, Porphyromonas gingivalis, Actinomyces viscosus, Leakage of intracellular proteins and DNAs after PAW exposure was observed through optical absorption. For safety study, PAW was incubated with oral epithelial cells for 10 minutes and the cell viability was detected by Cell Counting Kit-8. Optical emission spectroscopy (OES) was used to evaluate the reactive species generated by PMJ operated in water. Effective inactivation under PAW treatment was all achieved within 10 minutes. The sterilization results suggest that PAW is a promising new type of mouthwash to prevent or treat dental caries and periodontal related diseases. Detailed experimental setup and results will be discussed at the conference.