Title :
Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter
Author :
Wu, T.-H. ; Chen, Y. ; Park, S.-Y. ; Hong, J. ; Teslaa, T. ; Zhong, J.F. ; Carlo, D. Di ; Teitell, M.A. ; Chiou, P.-Y.
Author_Institution :
Univ. of California, Los Angeles (UCLA), Los Angeles, CA, USA
fDate :
Jan. 29 2012-Feb. 2 2012
Abstract :
We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20,000 mammalian cells/s with 37% sorting purity, 90% cell viability in enrichment mode, and >;90% purity in high purity mode at 1,500 cells/s or 3,000 beads/s. Fast switching (30 μs) and a small perturbation volume (~90 pL) is realized by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.
Keywords :
bioMEMS; cellular biophysics; fluorescence; high-speed optical techniques; laser beam applications; microfluidics; PDMS channel; PLACS; cell viability; mammalian cells; pulsed laser triggered fluorescence activated cell sorter; sorting purity; Fluorescence; Lasers; Microfluidics; Optical switches; Sorting; Throughput;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on
Conference_Location :
Paris
Print_ISBN :
978-1-4673-0324-8
DOI :
10.1109/MEMSYS.2012.6170196
