Title :
Diode-pumped lasers for two-photon fluorescence microscopy
Author :
Robertson, G. ; Armstrong, David ; Dymott, M.J.P. ; Ferguson, A.I. ; Hogg, Graeme
Author_Institution :
Dept. of Phys. & Appl. Phys., Strathclyde Univ., Glasgow, UK
Abstract :
Summary form only given. We report the use of diode-pumped mode-locked lasers for generating two-photon fluorescence microscopy (TPFM) images. TPFM offers several advantages over the more conventional confocal fluorescence microscopy, namely improved depth penetration through samples, confinement of dye bleaching to the focus area, and a reduction in the background fluorescence levels caused by out-of-focus fluorescence. In addition, the absorption confinement reduces phototoxicity for live samples.
Keywords :
fluorescence; laser beam applications; optical microscopy; optical pumping; solid lasers; two-photon processes; absorption confinement; background fluorescence; depth penetration; diode-pumped mode-locked laser; dye bleaching; live sample; phototoxicity; two-photon fluorescence microscopy; Diodes; Fluorescence; Laser mode locking; Laser theory; Microcavities; Microscopy; Power system reliability; Semiconductor lasers; Surface emitting lasers; Vertical cavity surface emitting lasers;
Conference_Titel :
Lasers and Electro-Optics, 1996. CLEO '96., Summaries of papers presented at the Conference on
Conference_Location :
Anaheim, CA, USA
Print_ISBN :
1-55752-443-2
