Title :
Multi-spectral tunable excitation fluorescence microscopy with a nanoimprinted PDMS-on-silicon grating optical filter
Author :
Huang, N.-T. ; Truxal, S. ; Tung, Y. -C ; Kurabayashi, K.
Author_Institution :
Univ. of Michigan, Ann Arbor, MI, USA
fDate :
Jan. 29 2012-Feb. 2 2012
Abstract :
We report a MEMS-based tunable grating optical filter device and its integration with a fluorescence microscope system. This integration allows for voltage-controlled wavelength tuning of excitation light from a multi-band source. We demonstrate the system´s capability to selectively excite fluorescence protein molecules with different excitation/emission spectral characteristics using a chip of optical filter device arrays, each with a very small (2mm × 2 mm) footprint. The demonstrated technology has potential to enable massively-parallel multispectral fluorescence microscopy in biochip array settings.
Keywords :
fluorescence; optical filters; optical microscopy; optical tuning; MEMS based tunable grating optical filter; biochip array settings; excitation/emission spectral characteristics; fluorescence microscope system; fluorescence protein molecules; multi spectral tunable excitation fluorescence microscopy; nanoimprinted PDMS on silicon grating optical filter; voltage controlled wavelength tuning; Fluorescence; Gratings; Laser excitation; Laser tuning; Optical filters; Optical imaging;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on
Conference_Location :
Paris
Print_ISBN :
978-1-4673-0324-8
DOI :
10.1109/MEMSYS.2012.6170270
