A MEMS-based approach to detection of single nucleotide polymorphisms for genetic disorder diagnosis

Author

Zhu, Jing ; Palla, Mirkó ; Ronca, Stefano ; Warpner, Ronald ; Ju, Jingyue ; Lin, Qiao

Author_Institution

Dept. of Mech. Eng., Columbia Univ., New York, NY, USA

fYear

2012

fDate

Jan. 29 2012-Feb. 2 2012

Firstpage

753

Lastpage

756

Abstract

This paper presents an integrated, MEMS-based approach to the detection of single nucleotide polymorphisms (SNPs). This SNP detection approach aims to eliminate complicated fluidic control operations by using a single-chamber microfluidic device, which incorporates polymerase chain reaction (PCR) and single base extension (SBE) on microbeads. Experimental results demonstrate that the device is capable of performing bead-based PCR, chemical elution, thermal elution, bead-based SBE, and on-chip desalting, which together potentially allow rapid, simple, accurate, multiplexed and highly efficient detection of SNPs.

Keywords

bioMEMS; genetics; microfabrication; microfluidics; polymorphism; MEMS-based approach; bead-based SBE; chemical elution; fluidic control operations; genetic disorder diagnosis; microbeads; microchip fabrication; on-chip desalting; polymerase chain reaction; single base extension; single nucleotide polymorphism detection; single-chamber microfluidic device; thermal elution; DNA; Fluorescence; Microfluidics; System-on-a-chip; Temperature control; Temperature measurement; Temperature sensors; Bead-Based Polymerase Chain Reaction; Genotyping; Microfluidics; Single Base Extension; Single Nucleotide Polymorphisms;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on

Conference_Location

Paris

ISSN

1084-6999

Print_ISBN

978-1-4673-0324-8

Type

conf

DOI

10.1109/MEMSYS.2012.6170295

Filename

6170295