Title :
A MEMS-based approach to detection of single nucleotide polymorphisms for genetic disorder diagnosis
Author :
Zhu, Jing ; Palla, Mirkó ; Ronca, Stefano ; Warpner, Ronald ; Ju, Jingyue ; Lin, Qiao
Author_Institution :
Dept. of Mech. Eng., Columbia Univ., New York, NY, USA
fDate :
Jan. 29 2012-Feb. 2 2012
Abstract :
This paper presents an integrated, MEMS-based approach to the detection of single nucleotide polymorphisms (SNPs). This SNP detection approach aims to eliminate complicated fluidic control operations by using a single-chamber microfluidic device, which incorporates polymerase chain reaction (PCR) and single base extension (SBE) on microbeads. Experimental results demonstrate that the device is capable of performing bead-based PCR, chemical elution, thermal elution, bead-based SBE, and on-chip desalting, which together potentially allow rapid, simple, accurate, multiplexed and highly efficient detection of SNPs.
Keywords :
bioMEMS; genetics; microfabrication; microfluidics; polymorphism; MEMS-based approach; bead-based SBE; chemical elution; fluidic control operations; genetic disorder diagnosis; microbeads; microchip fabrication; on-chip desalting; polymerase chain reaction; single base extension; single nucleotide polymorphism detection; single-chamber microfluidic device; thermal elution; DNA; Fluorescence; Microfluidics; System-on-a-chip; Temperature control; Temperature measurement; Temperature sensors; Bead-Based Polymerase Chain Reaction; Genotyping; Microfluidics; Single Base Extension; Single Nucleotide Polymorphisms;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on
Conference_Location :
Paris
Print_ISBN :
978-1-4673-0324-8
DOI :
10.1109/MEMSYS.2012.6170295
