A micro device for measuring single-cell membrane specific capacitance and cytoplasm conductivity

Author

Tan, Qingyuan ; Chen, Jian ; Zheng, Yi ; Chen, Brandon K. ; Sun, Yu

Author_Institution

Adv. Micro & Nanysosytems Lab., Univ. of Toronto, Toronto, ON, Canada

fYear

2012

fDate

Jan. 29 2012-Feb. 2 2012

Firstpage

757

Lastpage

760

Abstract

This paper presents a micro device using micro electrical impedance spectroscopy (μEIS) to extract single-cell membrane specific capacitance and cytoplasm conductivity. Single cells were aspirated and stopped inside a wedge-shaped constriction channel where their impedance profiles were recorded by an impedance analyzer. An equivalent circuit model was developed to extract cell electrical parameters from the measured impedance profiles. The system was applied to quantify membrane specific capacitance (4.48±1.17 μF/cm², n=11) and cytoplasm conductivity (0.59±0.33 S/m, n=11) of PAVICs (porcine aortic valve interstitial cells).

Keywords

bioMEMS; biomedical equipment; biomedical measurement; biomembranes; cellular biophysics; electrical conductivity; equivalent circuits; micromechanical devices; microorganisms; cell electrical parameters; cytoplasm conductivity; equivalent circuit model; microdevice; microelectrical impedance spectroscopy; single-cell membrane specific capacitance; wedge-shaped constriction channel; Capacitance; Cells (biology); Conductivity; Equivalent circuits; Glass; Impedance; Integrated circuit modeling;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on

Conference_Location

Paris

ISSN

1084-6999

Print_ISBN

978-1-4673-0324-8

Type

conf

DOI

10.1109/MEMSYS.2012.6170296

Filename

6170296