A study of cancer cell metastasis using microfluidic transmigration device

Author

Fu, Y. ; Vandongen, A.M.J. ; Bourouina, T. ; Park, W.T. ; Je, M.Y. ; Tsai, J.M. ; Kwong, D.L. ; Liu, A.Q.

Author_Institution

Sch. of Electr. & Electron. Eng., Nanyang Technol. Univ., Singapore, Singapore

fYear

2012

fDate

Jan. 29 2012-Feb. 2 2012

Firstpage

773

Lastpage

776

Abstract

Metastasis of cancer cell was studied using microfluidic device. Migration of cells in the microchannel array mimics their transmigration in tissue capillaries during in vivo metastasis. Details of the morphological changes were recorded through high resolution imaging. Results showed that the deformation of cell nucleus is critical for the transmigration of breast cancer cell MDA-MB-231. Condensation of chromatin by drug plicamycinon significantly impaired their deformation abilities and subsequently reduced the transmigration by 2 to 3 fold for microchannel smaller than 10 μm × 5 μm. As transmigration is critical for cancer metastasis, the results will be useful in designing new anti-cancer therapy.

Keywords

biological tissues; biomembrane transport; cancer; capillarity; cellular biophysics; drugs; microfluidics; MDA-MB-231; anti-cancer therapy; breast cancer cell; cancer cell metastasis; cell migration; cell nucleus; chromatin; condensation; drug; high resolution imaging; microchannel array; microfluidic device; microfluidic transmigration device; morphological changes; plicamycinon; tissue capillaries; Arrays; Breast cancer; Drugs; Metastasis; Microchannel; Microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on

Conference_Location

Paris

ISSN

1084-6999

Print_ISBN

978-1-4673-0324-8

Type

conf

DOI

10.1109/MEMSYS.2012.6170300

Filename

6170300