In situ confocal Raman spectroscopy of single living Chinese hamster ovary cells grown on different substrates

Author

Rodaite-Riseviciene, Raminta ; Snopok, Boris ; Snitka, Valentinas

Author_Institution

Res. Center for Microsyst. & Nanotechnol., Kaunas Univ. of Technol., Kaunas, Lithuania

fYear

2013

fDate

10-13 Nov. 2013

Firstpage

7

Lastpage

10

Abstract

Single living Chinese hamster ovary cells on the surface of different substrates have been characterized in-vivo by confocal scanning Raman spectroscopy and conventional optical microscopy. It was shown that both morphology and vibration fingerprint of cells depend on the nature of the substrate material (glass, silicon, bismuth germinate, quartz, lithium niobate, gold, silver, tin dioxide) and related to the specific features of the biochemical status of the cells within interaction area influenced by the growth conditions.

Keywords

Raman spectroscopy; biochemistry; biomedical measurement; bismuth compounds; cellular biophysics; glass; gold; lab-on-a-chip; lithium compounds; optical microscopy; quartz; silicon; silver; titanium compounds; Ag; Au; Bi₄Ge₃O₁₂; LiNbO₃; Si; SiO₂; TiO₂; cell biochemical status; cell morphology; cell vibration fingerprint; confocal scanning Raman spectroscopy; conventional optical microscopy; growth condition; in situ confocal Raman spectroscopy; interaction area; single living Chinese hamster ovary cells; substrate material; substrate surface; Glass; Gold; Proteins; Raman scattering; Silicon; Substrates; Surface morphology; cell adhesion; cell-on-chip microdevices; confocal Raman spectroscopy; single living cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Molecular Medicine and Engineering (NANOMED), 2013 IEEE 7th International Conference on

Conference_Location

Phuket

Print_ISBN

978-1-4799-2689-3

Type

conf

DOI

10.1109/NANOMED.2013.6766306

Filename

6766306