A novel multi-cue based image fusion method combining multi-principle microscopy images towards Nuclear transfer technology

Since its invention, the Nuclear transfer technology has a wide range of applications in fields such as animal breeding, gene therapy, organ transplants, etc.. However, since the oosight genetic material is not visible, presently the nucleus removal process of recipient oocyte always take the blind suction method, which led to extremely low success rate of nuclear transfer operation. Polarized light microscopy imaging system is very suitable for non -invasive imaging of living oocyte spindle. However, comparing to inverted microscopic imaging, it lost a lot of operating environment information (such as precision positioning of manipulation tools and cellular texture). In this paper, the advantages of polarized light microscopy imaging systems and traditional optical inverted microscope imaging system are combined. The fusion of images from polarized light microscopy imaging systems and traditional optical inverted microscope imaging system was realized. Collecting the polarized light microscopy image and inverted microscope image of same oosights in real time using two CCD cameras. A multi-cue image fusion method is proposed in this manuscript, experimental results show that this method is expected to achieve real-time visualized operation and observation.