Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor

Author

Weiss, Steven ; Ha, Taehoon ; Enderle, T. ; Ogletree, D.F. ; Selvin, P.R. ; Chemla

Author_Institution

Div. of Mater. Sci., Lawrence Berkeley Lab., CA, USA

fYear

1996

fDate

7-7 June 1996

Firstpage

25

Abstract

Summary form only given. Fluorescence resonance energy transfer (FRET) is a standard spectroscopic technique for measuring distances in the 10-75 /spl Aring/ range. We extended the technique´s sensitivity by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy (NSOM) was used to obtain simultaneous dual color imaging and emission spectra from a single pair of donor and acceptor fluorophores linked by a short (10 or 20 bases long) DNA molecule.

Keywords

DNA; biological techniques; fluorescence; infrared spectra; molecular biophysics; optical microscopy; 10 to 15 A; DNA molecule; FRET; NSOM; dual color imaging; emission spectra; energy transfer; fluorescence resonance energy transfer; near-field scanning optical microscopy; sensitivity; single acceptor; single acceptor fluorophore; single donor; single donor fluorophore; single molecules; standard spectroscopic technique; Color; Energy exchange; Energy measurement; Fluorescence; Measurement standards; Optical microscopy; Optical sensors; Resonance; Spectroscopy; Stimulated emission;

fLanguage

English

Publisher

ieee

Conference_Titel

Quantum Electronics and Laser Science Conference, 1996. QELS '96., Summaries of Papers Presented at the

Conference_Location

Anaheim, CA, USA

Print_ISBN

1-55752-444-0

Type

conf

Filename

865521