Synthesis and characterization of silver nanoparticles from Rhodococcus-2891 and its anti tumor activity against Lung cancer cell line (A549)

Rhodococcus is a genus of aerobic, non-sporulating, non-motile gram positive bacteria closely related to mycobacteria and corynebacteria. Strains of Rhodococcus contain enzymes that carry out biologically relevant reactions such as bio desulfurization of fossil fuels, degradation of Poly chlorinated Biphenyls (PCB) and utilizing a wide variety of other organic compounds as energy sources. It was commercially used as biocatalyst in production of fossil fuels, bioactive steroids and acrylamide. The synthesis and characterization of silver nanoparticles are determined by the treatment of aqueous solution 0.01 Millimolar silver nitrate with bacterial strain and silver nanoparticles could be fabricated at intracellular level and was characterized by UV-Spectroscopy. The size and shape of silver nanoparticles was investigated by using Transmission Electron Microscope (TEM). Fourier Transform Infrared Spectroscopy (FTIR) used to demonstrate the chemical nature of nanoparticles. The size range or mean of the particles in powder sample was determined by Particle Size Analyzer (PSA). Anti Cancerous activity was analyzed by MTT Assay and Apoptosis detection Hoechst 33342 DNA Staining. DNA sequences was amplified by Polymerase Chain Reaction (PCR).